Antibody validation, benchmarking and quantitative expression (CD Maps)

Kužílková D, Puñet-Ortiz J, Aui PM, et al.
Standardization of Workflow and Flow Cytometry Panels for Quantitative Expression Profiling of Surface Antigens on Blood Leukocyte Subsets: An HCDM CDMaps Initiative.
Front Immunol. (2022) 13:1–15. doi: 10.3389/fimmu.2022.827898

Kalina T, Lundsten K, Engel P.
Relevance of Antibody Validation for Flow Cytometry.
Cytometry A (2020) 97:126–136. doi:10.1002/cyto.a.23895

Kalina T.
Reproducibility of Flow Cytometry Through Standardization: Opportunities and Challenges.
Cytom Part A (2020) 97:137–147. doi:10.1002/cyto.a.23901

Kalina T, Fišer K, Pérez-Andrés M, Kuzílková D, Cuenca M, Bartol SJWW, Blanco E, Engel P, van Zelm MC.
CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets.
Front Immunol (2019) 10:2434. doi:10.3389/fimmu.2019.02434

Engel P, Boumsell L, Balderas R, Bensussan A, Gattei V, Horejsi V, Jin B-Q, Malavasi F, Mortari F, Schwartz-Albiez R, et al.
CD Nomenclature 2015: Human Leukocyte Differentiation Antigen Workshops as a Driving Force in Immunology.
J Immunol (2015) 195:4555–63. doi:10.4049/jimmunol.1502033

HLDA10 Research Publications

Special Issue of Clinical & Translational Immunology

Clark G, Stockinger H, Balderas R, van Zelm MC, Zola H, Hart D, Engel P.
Nomenclature of CD molecules from the Tenth Human Leucocyte Differentiation Antigen Workshop. Clin Transl Immunology.
2016 Jan 22;5(1):e57. doi:10.1038/cti.2015.38.

Clark GJ, Kupresanin F, Fromm PD, Ju X, Muusers L, Silveira PA, Elgundi Z, Gasiorowski RE, Papadimitrious MS, Bryant C, Lee KM, Clarke CJ, Young JW, Chan A, Harman A, Botting R, Cabezón R, Benitez-Ribas D, Brooks AE, Dunbar PR, Hart DN.
New insights into the phenotype of human dendritic cell populations. Clin Transl Immunology. 2016 Jan 29;5(1):e61. doi: 10.1038/cti.2015.40.

Ohradanova-Repic A, Machacek C, Fischer MB, Stockinger H.
Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel. Clin Transl Immunology. 2016 Jan 8;5(1):e55. doi: 10.1038/cti.2015.39.

Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ.
Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 Workshop in Wollongong, Australia. Clin Transl Immunology. 2015 Nov 13;4(11):e50. doi: 10.1038/cti.2015.29.

Gurka S, Dirks S, Photiadis J, Kroczek RA.
Expression analysis of surface molecules on human thymic dendritic cells with the 10th HLDA Workshop antibody panel. Clin Transl Immunology. 2015 Oct 30;4(10):e47. doi: 10.1038/cti.2015.21.

Filatov A, Kruglova N, Meshkova T, Mazurov D.
Lymphocyte phosphatase-associated phosphoprotein proteoforms analyzed using monoclonal antibodies. Clin Transl Immunology. 2015 Oct 9;4(10):e44. doi: 10.1038/cti.2015.22.

HLDA9 Research Publications

Special Issue of Immunology Letters

Matesanz-Isabel J, Sintes J, Llinàs L, de Salort J, Lázaro A, Engel P.
New B-cell CD molecules.
Immunol Lett. 2011 Jan 30;134(2):104-12. Epub 2010 Oct 7. PubMed PMID: 20933010.

Llinàs L, Lázaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P.
Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
Immunol Lett. 2011 Jan 30;134(2):113-21. Epub 2010 Oct 15. PubMed PMID: 20951740.

Rodríguez-Bayona B, Ramos-Amaya A, Brieva JA.
Differential expression of slams and other modulatory molecules by human plasma cells during normal maturation.
Immunol Lett. 2011 Jan 30;134(2):122-8. Epub 2010 Oct 13. PubMed PMID: 20923684.

De Salort J, Sintes J, Llinàs L, Matesanz-Isabel J, Engel P.
Expression of SLAM (CD150) cell-surface receptors on human B-cell subsets: From pro-B to plasma cells.
Immunol Lett. 2011 Jan 30;134(2):129-36. Epub 2010 Oct 7. PubMed PMID: 20933013.

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